Secondary Antibodies
At Pronewbio, we deeply understand that the reliability of your secondary antibody is the ultimate determining factor in achieving an optimal signal-to-noise ratio for any critical immunoassay. In an era where researchers are rapidly advancing fields such as oncology, neurological disease modeling, and complex organoid characterization, there is zero tolerance for background interference or unexpected cross-reactivity. That is why our comprehensive portfolio of secondary antibodies is meticulously engineered and rigorously validated in-house to provide unmatched sensitivity and absolute specificity.
Whether you are detecting low-abundance targets in dense tissue microenvironments, performing complex multi-color multiplexing with our advanced VHH single-domain antibodies, or executing routine applications including Western Blotting (WB), Immunofluorescence (IF), and Flow Cytometry, our reagents are built to deliver publication-ready, reproducible data. We have optimized our proprietary manufacturing and cross-adsorption pipelines to ensure strict lot-to-lot consistency. By bridging the gap between basic molecular biology and advanced preclinical diagnostics, Pronewbio empowers your transition from bench research to translational breakthroughs with complete analytical confidence.
Classification by Host Species and Target Specificity
Our secondary antibodies are developed in diverse host species including Goat, Rabbit, Donkey, Mouse, and Alpaca. We offer rigorous cross-adsorption processes to minimize non-specific binding in multiplexing experiments.
- Anti-IgG (H+L): Targets both the heavy and light chains for maximum signal intensity.
- F(ab')2 Fragment: Ideal for tissues rich in Fc receptors to eliminate background interference.
- Subclass Specific: High-precision targeting for Mouse IgG1, IgG2a, or IgG2b.
- VHH Antibodies: Single-domain recombinant antibodies derived from Alpaca, offering superior tissue penetration, high affinity, and exceptional stability.
- Antisera: Unpurified whole serum providing robust, broad-spectrum target recognition for complex preliminary assays.
Detection Systems and Conjugates
We provide a comprehensive range of labels tailored to your specific detection platforms and multiplexing requirements:
- Enzymatic: HRP, AP, and Beta-Galactosidase.
- Classic Fluorophores: FITC, TRITC, and Texas Red.
- Advanced Fluorophores: Cyanine dyes including Cy3, Cy5, and Cy7.
- Phycobiliproteins: PE (Phycoerythrin) and APC (Allophycocyanin).
- Biotinylated: Designed for robust amplification via Avidin/Streptavidin complexes.
- Colloidal Gold: Optimized for electron microscopy and rapid lateral flow applications.
- Solid Phase Supports: Agarose and Magnetic Beads for efficient immunoprecipitation.
Technical Specifications and Performance Data
1: Feature & Specification Details
- Concentration: Typically 1 mg/mL to 2 mg/mL
- Purification: Affinity chromatography (Antigen-specific)
- Storage Buffer: PBS with stabilizing proteins and 0.05% Sodium Azide
- Shelf Life: 12 to 24 months at -20°C
- Cross-Adsorption: Available against Human, Rat, and Bovine serum proteins
2: Optimized Dilution Ratios
To ensure the best results, we recommend the following starting points for your optimization:
- WB (HRP): 1:5000 to 1:50000
- IHC/IF: 1:200 to 1:1000
- ELISA: 1:10000 to 1:100000
Case Study
High-contrast immunofluorescence imaging of cellular membranes demonstrates the exceptional target specificity and negligible background noise achieved using Pronewbio fluorescently conjugated secondary antibodies, counterstained with DAPI for distinct nuclear visualization.
Why Choose Pronewbio Secondary Antibodies?
As a leader in the biotech space, Pronewbio ensures that every lot undergoes stringent QC testing. Our antibodies are validated in-house using complex cell lysates to guarantee that your results are reproducible and publication-ready.
- High Sensitivity: Detect low-abundance proteins at concentrations as low as 10 M cells per sample.
- Low Background: Pre-adsorbed options ensure zero cross-reactivity in multi-color labeling.
- Global Compliance: All products are indexed and searchable, ensuring full transparency and traceability.