To accelerate the screening of expansive antibody libraries during early-stage drug development, Pronewbio Biotech Ltd. offers a proprietary High-Throughput (HTP) recombinant expression service. Our system is specifically engineered to bridge the gap between initial sequence discovery and functional characterization, enabling the rapid production of hundreds of unique antibody candidates in less than 14 days.

Core Technical Advantages

By integrating our in-house proEM high-expression vector suite with optimized mammalian host systems, we provide a streamlined solution for large-scale antibody production.

• High Productivity: Capable of processing up to 1000 individual antibody constructs per month.
• Rapid Delivery: Expedited workflows facilitate purified protein delivery in as little as 2 weeks.
• Format Versatility: Our platform supports Full-length IgG, Fab fragments, scFv, VHH (Nanobodies), and Bispecific constructs.
• Mammalian Excellence: Primary utilization of suspension-adapted CHO and HEK293 cells for superior post-translational fidelity.

Streamlined Operational Workflow

Our platform utilizes an automated, parallel-processing pipeline to ensure consistency across every batch.

1. Sequence Synthesis and mandatory validation: Every project begins with optimized codon usage and sequence verification to ensure 100 percent genetic accuracy.
2. Automated Vector Construction: High-efficiency cloning into our proprietary proEM expression vectors.
3. HTP Plasmid Preparation: Transfection-grade plasmid extraction performed in parallel.
4. Transient Transfection: High-density mammalian cell transfection (scales ranging from 1mL up to 100mL).
5. Parallel Purification: One-step affinity chromatography tailored for HTP throughput.
6. Analytical Quality Control: Verification of concentration, purity, and optional endotoxin levels.

Platform Performance Specifications

• Host Cell Lines: HEK293-S / CHO-S (Chemically defined, serum-free)
• Throughput Capability: Up to 1000 single clones per month
• Production Scale: Flexible volumes from 1mL, 5mL, 10mL, to 20mL per construct
• Genetic Verification: Mandatory sequence validation to ensure 100 percent fidelity
• Purity Benchmark: Greater than 90 percent as determined by SDS-PAGE
• Deliverables: Purified antibody, Certificate of Analysis (CoA), and verified plasmid maps

Supporting Translational Discovery

The high-throughput expression platform is a critical component of our integrated antibody discovery services, which include SingleB B-cell screening and professional humanization. Whether you are developing chimeric antibodies for oncology or exploring fully humanized candidates for neurological disorders, our platform provides the scalability and speed required to identify lead molecules with therapeutic potential.

Case Study

Production of Anti-Amyloid Beta 1-5 Antibody, Clone 3D6 (Mouse IgG1 kappa)